26.09.2016

Nanodrop spectrophotometer

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Nanodrop ND-1000 spectrophotometer

NanoDrop.jpg

Nanodrop ND-1000 spectrophotometer. (Picture taken by Saku Mattila.)

Name and Model (Year of Manufacture / Installation)

Thermo Scientific Nanodrop ND-1000 (2005/2005)

General Information

Spectrophotometer for analyses of protein, cell density measurements, and measuring  the nucleic acid concentration and purity of nucleic acid samples.

Spektrofotometri.

Key Specifications
  • Sample size: 1 microliter
  • Path Length: 1 mm (with auto-ranging to 0.2 mm)
  • Detector Type: 2048-element linear silicon CCD array
  • Wavelength Range: 220-750 nm
  • Wavelength Accuracy: 1 nm
  • Wavelength Resolution: 3 nm (FWHM at Hg 546 nm)
  • Absorbance Precision: 0.003 absorbance (1mm path)
  • Absorbance Accuracy: 2% (at 0.76 absorbance at 257 nm)
  • Absorbance Range: 0.02-75 (10 mm equivalent absorbance)
  • Detection Limit: 2 ng/microliter (dsDNA)
  • Maximum Concentration: 3700 ng/microliter (dsDNA)
  • Measurement Cycle Time: 10 seconds
  • Dimensions: 14 cm X 20 cm

Light Source: Xenon flash lamp
Sample Pedestal Material of Construction: 303 stainless steel and quartz fiber

Key Features

Application modes

  • Nucleic Acid – concentration and purity of nucleic acid
  • MicroArray – dye incorporation concentration and purity of nucleic acid
  • UV-Vis – general UV-Vis measurements
  • Cell Cultures – “absorbance” (light scattering) measurement of suspended microbial cells
  • Protein A280 – concentration and purity of purified protein
  • Proteins & Labels – concentration of dye-labeled proteins, conjugates, and metalloproteins 3-5 Section 3-General Operation
  • Protein BCA – protein concentration using the BCA assay
  • Protein Bradford – protein concentration using the Bradford assay • Protein Lowry – protein concentration using the Modified Lowry assay
  • Pierce 660 nm Protein Assay – protein concentration using the new 660 nm assay

Software: Data Viewer.

Location, Responsible Person

Department of Biological and Environmental Science; Ambiotica, YAB 224.1 / Alli Liukkonen