Perkin Elmer Series 200 HPLC

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Perkin Elmer Series 200 Liquid Chromatography (HPLC) equipment


Perkin Elmer 200 series liquid chromatography (HPLC) system. (Picture taken by Saku Mattila.)

Name and Model (Year of Manufacture/Installation)

Perkin Elmer series 200 HPLC (2003/2003)

General Information

Analytical liquid chromatography (LC) equipment. A high performance liquid chromatograph (HPLC).

Analyyttinen nestekromatografialaitteisto yhdisteiden tutkimiseen.           

Key Specifications       

Parts of the system:

  • Perkin Elmer Series 200 pump
  • Perkin Series 200 Peltier column oven
  • Perkin Series 200 Refractive Index (RI) Detector
  • Perkin Series 200 UV/Vis Detector
  • Perkin Elmer 600 Link device
  • Perkin Elmer 900 Network Chromatography Interface
  • (and Perkin Elmer Series 200 Degasser)

UV/Vis detector

Wavelength range:

190-700 nm


5 nm

Wavelength accuracy:

+/- 1 nm


Dual beam

Sensitivity Range:

0.0005 to 3.000 AUFS in 0.0001 increments from 0.0005 to 0.1, and 0.01 increments above 0.1 AUFS.

Recorder output:

10 mV full scale for any selected range.

Computer output

1 V/AU, 0.5 V/AU, 0.2 V/AU (software control)


< +/- 1 x 10 -5 AU, 210-280 nm, 1 sec. rise time, test flowcell.


< 1 x 10 -4 AU/hour, after warm-up

Light sources:

Deuterium (190-360nm) or Tungsten (360-700nm) prefocused-no adjustment required on replacement.

Leak detection:

Heated thermistor sensor in glass envelope located in flow cell drip pan


Graphic LCD module 240 X 64 pixel. Series 200 standard

Noise filter:

Digitally controlled active filter with response (RSP) or peak width (PW) selectable from 0.02 to 5.0 seconds in 8 steps.

Number of methods:

20 in battery backed memory

Steps per method:

20 maximum; multiple simultaneous events may occur in any given step

Method time:

Up to 999.99 minutes per method

Programmable functions from

the front keypad or Turbochrom:

Time, wavelength, response time, auto-zero, relay closures, method number, number of injections, (method execution via sequence)


Stopped flow scan, scan speed selectable from 0.2 to 1.0 nm/sec

External communications:

RS-232 for control, down load of methods via Turbochrom and upload of chromatographic data. Max. Chromatographic data rate is 50 data points per second.


Self-testing as per software diagnostic standards


Peltier Column oven



Operating Range (Heat only units):

30 ˚C to 90 ˚C

Operating Range (Peltier units):

5 ˚C to 90 ˚C (max 15 ˚C below ambient)

Operating Range (Oven/col select units):

7 ˚C to 90 ˚C (max 15 ˚C below ambient)

Recommended Flow Range:

Up to 5 mL/min

Temperature Accuracy:      

± 1 ˚C over entire range

Temperature Stability:

±0.2 ˚C

Temperature Repeatability:

± 1 ˚C

Heating Rate (Heat only units):

30 min to setpoint at 2mL/min

Heating / Cooling Rate (Peltier units):

30 min to 10 ˚C or 60 ˚C at 2ml/min

Temperature Ramp:

Up to 5 ˚C/min

Stabilizing Time:

30 min after reaching setpoint

Column Selection Valve Capacity:

6 switching positions


Refractive Index (RI) detector

Light source: Tungsten halogen lamp
Range (RIU): 1.00-1.75
Detection type: Deflection-microprocessor control
Remote operations: Zero/polarity/purge  
Flow cell: 8-µL volume,  483 kPa (70 psi) backpressure rating


Perkin Elmer 200 series Pump

Flow rate range: 0.01 to 10.0 mL/min (maximum pressure of 420 bar (6200 psi) over the entire range)

Flow rate increments: 0.01 from 0 to 0.99 mL/min, 0.1 from 1 to 10 mL/min
Flow precision: 0.3% relative standard deviation (typically <0.1%) at 1 mL/min and 70 bar (1000 psi) with water as the mobile phase
Flow accuracy: ± 1% of setting at 1ml/min and 70 bar (1000 psi) with water as the mobile phase
Retention time reproducibility: <0.3% RSD (typically <0.1% RSD)
Pressure range: 0 to 420 bar (6200 psi) from the entire flow-rate range
Pressure limits: High and low limits user-settable to 1 bar (10 psi) increments

Solvent compressibility: Keyboard settable flow calibration
Composition range: 0 to 100% solvent A-D (depending on the pump configuration)
Composition settability: Settable to 0.1%
Composition accuracy: ± 1.0% absolute from 3 to 97% at 5 ml/min
Composition precision: Typically less tan 0.2% varation
Gradient curves: Linear, exponential 1 to 9.9 (positive and negative), or step (89 profile selections)
Linearity: Within ±1.0 % from 10 to 90 % at 5 mL/min

Program steps: Step 0 plus up to 20 solvents program steps. Battery-backed storage up to 30 methods plus one default method.
Step time: 0 to 999 min
Step time increments: 0.1 min, 0 to 9.9 min; 1.0 min 10 to 99 min
Programmed time events: Two built-in independent momentary (two second) contact closures
Events/methods: Maximum of 20
Event increments: 0.1 min, 0 to 9.9 min; 1.0 min 10 to 99 min

Key Features
  • No autosampler.

Refractive index detector:

  • Internal temperature control of the flow cell
  • Offset adjustment
  • Autozero
  • Autopurge of the reference cell
  • Polarity marker
  • Leak Drain
Location, Contact Person

Dept. of Chemistry, YSK338 / Jukka Pekka Isoaho

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