26.09.2016

Waters 996 HPLC

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Waters 996 High Performance Liquid Chromatography (HPLC) system

Waters996.jpg

 Waters 996 HPLC system. (Picture taken by Saku Mattila.)

Name and Model (Year of Manufacture / Installation)

Waters 996 HPLC system (?/?)

General Information

A high performance liquid chromatography (HPLC) system. The equipment is used for analyzing and characterizing the compounds. The system can perform standard liquid chromatography analysis and also size-exclusion chromatography (SEC) (or gel permeation chromatography (GPC)).

Key Specifications        

Parts of the system:

  • Waters 996 Liquid Chromatography unit
  • Waters 996 Photodiode Array (PDA) Detector
  • Waters 410 Differential Refractometer
  • Waters System Interface Module
  • Polymer Laboratories PL-ELS-1000 Evaporative Light Scattering Detector
  • Waters 717plus Autosampler
  • Waters Temperature Control Module
  • 2 pumps

Waters 996 Phodiode Array Detector

 

Wavelength range: 190 to 800 nm
Wavelength accuracy: ± 1 nm
Linearity range (Per ASTM 685-79): 5 % at 2.0 AU (probylparaben at 258 nm)
Spectral resolution: 1.2 nm
Baseline noise: ± 1.5 x 10-5 AU peak-to-peak, dry (at 254 nm)
Drift: 1 x 10-3 AU/hour (at 254 nm, after warmup)

Flow cells                        Path length (mm)               Tube inner diameter (inch)

Standard                             10                                       0.009 (0.23 mm)                
Semi-preparative                 3                                         0.040 (1.016 mm)
Microbore                            3                                         0.005 (0.127 mm)
Inert                                    10                                        0.001 (0.254 mm)

Polymer Laboratories PL-ELS-1000 Evaporative Light Scattering Detector

 

  • Light source: Tungsten/Halogen lamp
  • Detector: Photodiode

Temperature range

  • Evaporator: 30 - 300 °C
  • Nebulizer: 30 - 220 °C

Nebulizer gas

  • Typical inlet pressure: 413 - 689 kPa (60 - 100 psi)
  • Maximum inlet pressure: 1 MPa (150 psi)
  • Gas flow rate: 0 - 1.0 SLM (standard litre per minute)
  • Eluent flow rate: 0 - 0.5 mL/min  
  • Analog output:  0 - 1 V and 0 - 10 V FSD

Waters 410 Differential Refractometer

 

Rl Range: 1.00 to 1.75 RIU

Measurement Range:
5x10-3 RIU maximum FS (SENS= 1, SF=1)
5 x 10-a RIU minimum FS (SENS=1024, SF=100)

Flow Rate: 0.3 - 10 mL/min

Noise: 2.0 x 10-8 RIU, (TC = 1, SF = 20, SENS = 32, THF at 24 °C with restrictor coil)

Drift: 2.5 x 10-7 RlU/hour (static with THF)

Sensitivity Settings: 1, 2, 4, 8, 16, 32, 64, 128, 256, 512, 1024

Time Constant Filter Settings: 0.2, 1, 3, 10 seconds

Recorder Output: 0 to 10 mV

Temperature Control:  

  • Internal oven: 30 to 50 °C
  • External column heaters: Ambient to 150 °C

Integrator output

Sensitivity setting                      Output (V)      

1                                                      1
2                                                      2
4                                                      4
8                                                      8
16 to 1024                                      10


Optical Component Specifications


Light Source: 930 nm pulsed Light Emitting Diode (LED)

Flow Cell: Fused quartz

Cell Volume: 10 μL

Pressure Limit: 689 kPa (100 psi)

Fluid Path Materials: 316 stainless steel, Dynasii®, Suprasil II®, Teflon®, Kalrez®, quartz.

Key Features

PL-ELS-1000 Evaporative Light Scattering Detector:

+ PL-ELS 1000 control software enables:

  • temperature and gas flow control
  • signal monitoring
  • methods storage
Location, Contact Person

Department of Chemistry, YSK338 / Hannu Pakkanen