Zeiss Cell Observer HS fluorescence microscope with TIRF

Zeiss Cell Observer HS fluorescence microscope with TIRF


Name and Model (Year of Manufacture / Installation)

Carl Zeiss Cell Observer HS (2009/2009)

General Information

High speed live cell imaging system with one-color TIRF.

Key Features

Inverted microscope equipped with

  • Colibri LED light source (365, 455, 470, 505, 590, 625 nm) and XBO lamp.
  • Zeiss Filter sets
    • 56HE eGFP/DsRed,
    • 59HE ECFP/EYFP,
    • 62 BFP/GFP/HcRed,
    • 60 CFP/YFP/HcRed,
    • 52 GFP (suitable for TIRF)
    • 64HE mCherry
    • 51 CFP
    • Two cameras: Zeiss AxioCam MRm (1388 x 1040 pix, pix size 6.45 µm) for high-resolution imaging and Rolera EM-C2TM (1004 x 1002 pixels, pix size 8 x 8 µm) for low light or high speed imaging.
    • Achroplan 20x (NA=0.45), Plan-Neofluar 40x (NA=0.6), Plan-Neofluar 63x (NA=1.2) water/glycerol immersion, Plan-Apochromat 63x (NA=1.4) Ph3 oil immersion and Plan-Apochromat 100x (NA=1.46) objectives.
    • DIC and phase contrast imaging possibility.
    • Large XL incubator with accurate CO2 and temperature feedback (Pecon).
    • Motorized stage for multipoint-imaging.
    • Definite focus system to correct focus drift.
    • TIRF (100x, NA=1.46, Plan-Apochromat objective, Filter set 52) with multiline Argon laser (458nm, 488nm and 514nm).
    • Aviovision Rel.4.8 software.
Location & Contact Person

Located at Nanoscience Center room YN045.3

Nina Rintanen
tel. +358 40 8053926 Ambiotica
room C214.2
Survontie 9, 40500 Jyväskylä