Two collaboration projects between NSC and BINA have started at 1.11.2018. These two projects are seed projects, where the opportunities for larger cooperation between NSC and BINA are explored. Both projects have got 20 000 € funding (reagents, materials, publication cost, etc.), half of which is paid from NSC and half from BINA. In addition, Erasmus funding can be used for mobility; for more information you will get form Leena Mattila (JYU).

Development of Nanoprobes Facilitating Sensitive Fluorescent Measurements in Cellular Vesicles

Varpu Marjomäki (NSC), Jussi Toppari (NSC), Rachela Popovtzer (BINA), and Dror Fixler (BINA)

Project Description: Intercalating dyes have commonly been used to detect DNA/RNA within a solution since their fluorescence is efficiently quenched before they connect to the DNA or RNA helix. Connection to helix makes them more rigid, which prevents the quenching and thus enables the fluorescence. These same dyes could be utilized inside a living cell also to detect nucleic acids within a vesicle, cytoplasm or inside nucleus. Recently, there has appeared a strong need to detect nucleic acids inside endocytic vesicles only. For that purposes, one needs to prevent the intercalating dyes to enter other parts of the cell, e.g. the nucleus and cytoplasm containing massive amounts of DNA and RNA, respectively. Another major obstacle in utilizing these dyes have been their low level of fluorescence. To tackle both of these obstacles simultaneously and thus enable sensitive detection of nucleic acids solely in cellular vesicles, we are developing new nanoprobes based on intercalating dyes and nanoparticles.

Examining Nuclear Dynamics of mRNA during Viral Infection

Maija Vihinen-Ranta (NSC) and Yaron Shav-Tal (BINA)

Project Description: The intra-nuclear movement of messenger RNA (mRNA) is a critical step both for the cellular and viral life cycles. The understanding of mRNA dynamics in cells is essential for basic research, and for the development of oncolytic virus therapy and novel antivirals that aim to block the nuclear steps of viral life cycle. We are focusing on initiating a collaboration between NSC and BINA in order to set up means by which to study the nuclear dynamics of mRNA in the absence and presence ofherpesvirus infection.